CGSC Strain#: 3509
Strain Designation: AB3509     
Source of Strain: J.A. Wechsler
Sex: F-
Chromosomal Markers: glnX44(AS)
,
galK2(Oc)
,
trpE3(Oc)
,
hisG4(Oc)
,
rpsL8 or rplL9(L?)
,
malT1(λ
R)
,
mtl-1,
ilvD144,
metE46,
ΔargH1,
thiE1Strain Comments: - glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- AS = amber(UAG) suppressor
- LamR = Lambda-resistant
- Oc = ochre(UAA) mutation
- strR = streptomycin resist.
References: - Wechsler, J.A. 1969. Antipolarity in the ilv operon of Escherichia coli K-12. J.Bacteriol. 98:1179-1194