CGSC Strain#: 3518
Strain Designation: AB3518     
Source of Strain: J.A. Wechsler
Sex: F-
Chromosomal Markers: leuB6(Am)
,
fhuA1,
Δ(gpt-proA)62,
lacY1 or lacZ4,
tsx-3,
galK2(Oc)
,
λ-,
trpE3(Oc)
,
hisG4(Oc)
,
malT1(λ
R)
,
mtl-1,
metE46,
thiE1,
sup-38(AS)
Strain Comments: - leuB6(Am)-- Suppressed by serU suppressor (supH)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- sup-38(AS)-- unmapped amber suppressor, may be supE44.
- Δ(gpt-proA)62 was formerly called proA2
- galK2(Oc) was formerly called gal2
- thiE1 was formerly called thi-1
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- LamR = Lambda-resistant
- Oc = ochre(UAA) mutation
References: - Wechsler, J.A. 1969. Antipolarity in the ilv operon of Escherichia coli K-12. J.Bacteriol. 98:1179-1194