CGSC Strain#: 14165
Strain Designation: χ1488/pGreenTIR      Source of Strain: A. Bochdansky
Other Designations: chi1488/pGreenTIR
Other Designations: GFP-minicells
Sex: F- Episome/Plasmid: pGreenTIR
   Plasmid Markers/Mutations: ori_pUC, bla(ApR)
   Plasmid Comment: The gfp allele in pGreenTIR has two amino acid substitutions (F64L and S65T) compared to wildtype resulting in increased protein solubility and causing a shift​ in the excitation maximum from 395 to 490 nm. The vector backbone is pUC1813.
Chromosomal Markers: purE41, glnV42(AS), λ^-, serC53, minB-2, his-53, xyl-14, metB65, cycA1, hsdR2, cycB2, ilv-277, tte-1
Strain Comments:

• minB-2-- mini-cell producer
• metB65-- is probably metB. Sometimes suggested to be metC.
• glnV42(AS) was formerly called supE42
• serC53 was formerly called pdxC3
• serC53 was formerly called serC3
• AS = amber(UAG) suppressor
• ApR = ampicillin resistant

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