CGSC Strain#: 4370
Strain Designation: KL236     
Source of Strain: M.C. Cappello
Sex: Hfr
Point of Origin#: 2A
Map Position: 12.20
Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: fhuA22,
phoA4(Am)
,
serS15(ts)
,
serS29,
ompF627(T2R)
,
serU132(AS)
,
fadL701(T2R)
,
relA1,
rrnB-2,
mcrB1,
creC510Strain Comments: - The strain is no longer temperature-sensitive for growth.
- phoA4(Am)-- is Garen's phoAS26 . It is suppressed by leuX151, glnV33(=supE33) and serU132(=supD32).
- serS15(ts)-- is a temperature-sensitive mutation.
- serS29-- is a second mutation resulting in partial restoration of the serS+ phenotype.
- rrnB-2-- : loss of spacer loop sequence
- creC510-- The constitutive phenotype is due to an R77P amino acid substitution
- creC510 was formerly called phoM510
- serU132(AS) was formerly called supD32
- serU132(AS) was formerly called Su1+
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- Const = constitutive
- T2R = T2 Resistant
- ts = Temperature Sensitive
References: - Low, B, F Gates, T Goldstein, D Soll 1971. Isolation and partial characterization of temperature-sensitive Escherichia coli mutants with altered leucyl- and seryl-transfer ribonucleic acid synthetases. J. Bacteriol. 108(2):742-50.