CGSC Strain#: 4381
Strain Designation: KL416     
Source of Strain: M.C. Cappello
Sex: F-
Chromosomal Markers: araC14,
leuB6(Am)
,
lacZ36,
proC32,
λ-,
hisF860(Stable)
,
cysJ43,
thyA54,
rpsL109(strR)
,
malA38,
xylA5,
mtl-1,
thiE1,
spc-15Strain Comments: - This strain carries an uncharacterized mutation near his which confers a Mal+ phenotype.
- leuB6(Am)-- Suppressed by serU suppressor (supH)
- cysJ43-- - the cys allele was formerly thought to be in cysC.
- thyA54-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
- malA38-- : In Χ65 a Mal+ phenotype may have resulted from suppression of malA1, but in the absence of evidence, we show the mutations as malA38 and sup-78.
- proC32 was formerly called proC65
- thiE1 was formerly called thi-1
- Am = amber(UAG) mutation
- Stable = stable, non-reverting
- strR = streptomycin resist.
References: - Lloyd, R.G., K.B. Low, G.N. Godson, E.A. Birge 1974. Isolation and characterization of an Escherichia coli K-12 mutant with a temperature-sensitive RecA- phenotype. J.Bacteriol. 120:407-415