CGSC Strain#: 4437
Strain Designation: Ftrp/7     
Source of Strain: Press & Maas
Sex: F'
Episome/Plasmid: F155
Plasmid Markers/Mutations: att$\phi$80 ,
trp Chromosomal Markers: thr-1,
leuB6(Am)
,
trp-1,
recA1,
rpsL119(strR)
,
ΔargH1,
thiE1,
φ80RStrain Comments: - This strain should be grown on medium containing thi, arg, leu, thr and lacking tryptophan. It should be grown at 30 degrees C.
- leuB6(Am)-- Suppressed by serU suppressor (supH)
- recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
- φ80R-- : unmapped φ80 resistance
- thiE1 was formerly called thi-1
- Am = amber(UAG) mutation
- strR = streptomycin resist.
References: - Press, R, N Glansdorff, P Miner, J De Vries, R Kadner, WK Maas 1971. Isolation of transducing particles of phi-80 bacteriophage that carry different regions of the Escherichia coli genome. Proc. Natl. Acad. Sci. U.S.A. 68(4):795-8.