CGSC Strain#: 4598
Strain Designation: W945     
Source of Strain: J. Lederberg
Sex: F-
Chromosomal Markers: thr-1,
araC14,
leuB6(Am)
,
lacY1,
glnX44(AS)
,
galK2(Oc)
,
malT1(λ
R)
,
xylA5,
mtl-1,
thiE1Strain Comments: - This should have been designated CGSC#4958. Only J. Cronan received this erroneous designation.
- leuB6(Am)-- Suppressed by serU suppressor (supH)
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- LamR = Lambda-resistant
- Oc = ochre(UAA) mutation
References: - Watson, J.D., W. Hayes 1953. Genetic exchange in Escherichia coli K-12; evidence for three linkage groups. Proc.Natl.Acad.Sci.USA 39:416-426