CGSC Strain#: 5214
Strain Designation: ND32      Source of Strain: E. Ohtsubo
Sex: F' Episome/Plasmid: F152-3
   Plasmid Markers/Mutations: Δ(kdpC-kdpA)18, Δ(ubiF-glnV)1000
   Plasmid Comment: Note the two deletions in this F152 derivative: ({\it kdpC-A} and {\it ubiF-glnV}).
Chromosomal Markers: Δ(nadA-galE)35, λ^-, recA1, relA1, rpsL180(strR), thiE1
Strain Comments:

• Note deletions on F152-3.
• recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
• thiE1 was formerly called thi-1
• strR = streptomycin resist.

References:

• Ohtsubo, E, MT Hsu 1978. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli: structure of F100, F152, and F8 and mapping of the Escherichia coli chromosomal region fep-supE-gal-attlambda-uvrB. J. Bacteriol. 134(3):778-94.