CGSC Strain#: 5814
Strain Designation: ASH1     
Source of Strain: I.B. Holland
Sex: Hfr
Point of Origin#: 129
Map Position: 8.60
Direction of Marker Transfer: Clockwise
Chromosomal Markers: Sfa(att)
7,
λ-,
thyA117,
rpsL228(strR)
,
metB1Strain Comments: - ASH1 transfers the chromosome predominantly from PO 129, but does give some transfer from PO 130.
- thyA117-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
- strR = streptomycin resist.
References: - Buxton, R.S., I.B. Holland 1973. Genetic studies of tolerance to colicin E2 in Escherichia coli K12. I. Re-location and dominance relationships of cet mutations. Mol.Gen.Genet. 127:69-88