CGSC Strain#: 6003
Strain Designation: AB6003      Source of Strain: E. Seeberg
Sex: F- Chromosomal Markers: thr-1, leuB6(Am), Δ(gpt-proA)62, lacY1, glnX44(AS), galK2(Oc), λ^-, uvrC34, thyA49, rpsL31(strR), argE3(Oc), deoB17, [lig-7](ts)
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• thyA49-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• [lig-7](ts)-- is a temperature-sensitive mutation which was brought in from E. coli 15; it reverts. The strain must be monitored frequently.
• Δ(gpt-proA)62 was formerly called proA2
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.
• ts = Temperature Sensitive

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