CGSC Strain#: 6126
Strain Designation: RH2-2     
Source of Strain: R.L. Hanson
Sex: F-
Chromosomal Markers: lacY1,
tsx-29,
glnX44(AS)
,
galK2(Oc)
,
λ-,
aroD6,
rpsL700(strR)
,
mtl-1,
pgi-2Strain Comments: - glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- pgi-2-- : pgi mutants utilize glucose by the hexose monoP shunt; strains with this allele grew on glucose at 1/3 parental rate. Slow growth on Gal,Mal,Lac;normal on Fru and gluconate.
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
- strR = streptomycin resist.
References: - Hanson, RL, C Rose 1979. Genetic mapping of a mutation affecting pyridine nucleotide transhydrogenase in Escherichia coli. J. Bacteriol. 138(3):783-7.