CGSC Strain#: 13661
Strain Designation: pLC34-20     
Source of Strain: Sex: F+
Episome/Plasmid: pLC34-20A
Plasmid Markers/Mutations: thr-1,
araC14,
leuB6(Am)
,
lacY1,
glnX44(AS)
,
galK2(Oc)
,
λ-,
mtl-1,
thiE1,
galT22,
recA56,
xylA5,
ΔtrpE63,
pyrH ,
tdi-?? ,
dnaE ,
proS ,
metD ,
fadE ,
rrnH ,
rrsH ,
alaV ,
ileV ,
rrlH ,
rrfH ,
aspU ,
dnaQ ,
rnhA ,
strC Plasmid Comment: Assumed to have all the genes between dnaE and dnaQ. Hybridizes with Kohara clones 121-123. Carries strC at 7.0 minutes. See also pLC34-20B.
Chromosomal Markers: thr-1,
araC14,
leuB6(Am)
,
lacY1,
glnX44(AS)
,
galK2(Oc)
,
galT22,
λ-,
ΔtrpE63,
recA56,
xylA5,
mtl-1,
thiE1Strain Comments: - leuB6(Am)-- Suppressed by serU suppressor (supH)
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- recA56-- Amino acid substitution Arg 60 Cys
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- ΔtrpE63 was formerly called DE(trpE5)
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
References: - Clarke, L, J Carbon 1976. A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome. Cell 9(1):91-9.
- Clarke, L., J. Carbon 1979. Selection of specific clones from colony banks by suppression or complementation tests. Methods Enzymol. 68:396-408
- Nishijima, M., C.E. Bulawa, C.H.R. Raetz 1981. Two interacting mutations causing temperature-sensitive phosphatidylglycerol synthesis in Escherichia coli membranes. J.Bacteriol. 145:113-131
- Horiuchi, T., H. Maki, M. Maruyana, M. Sekiguchi 1981. Identification of the dnaQ gene product and location of the structural gene for RNase H of Escherichia coli by cloning of the genes. Proc.Natl.Acad.Sci.USA 78:3770-3774
- Nishimura, A., K. Akiyama, Y. Kohara, K. Horiuchi 1992. Correlation of a subset of the pLC plasmids to the physical map of Escherichia coli K-12. Microbiol.Rev. 56:137-151