CGSC Strain#: 13843
Strain Designation: pLC13-12      Source of Strain:
Sex: F+ Episome/Plasmid: pLC13-12A
   Plasmid Markers/Mutations: thr-1, araC14, leuB6(Am), lacY1, glnX44(AS), galK2(Oc), λ^-, mtl-1, thiE1, galT22, recA56, xylA5, ΔtrpE63, uvrC , tyrP , fliA
   Plasmid Comment: Mixed clone. Hybridizes with Kohara clones 410-411 ca. 53 min, and carries uvrC, tyrP, and fliA ca. 43 min. See also pLC13-12B.
Chromosomal Markers: thr-1, araC14, leuB6(Am), lacY1, glnX44(AS), galK2(Oc), galT22, λ^-, ΔtrpE63, recA56, xylA5, mtl-1, thiE1
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• recA56-- Amino acid substitution Arg 60 Cys
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• ΔtrpE63 was formerly called DE(trpE5)
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation

References:

• Clarke, L, J Carbon 1976. A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome. Cell 9(1):91-9.
• Silverman, M, P Matsumura, M Hilmen, M Simon 1977. Characterization of lambda Escherichia coli hybrids carrying chemotaxis genes. J. Bacteriol. 130(2):877-87.
• Van Sluis, C.A., H. Pannekoek 1978. J.Supramol.Struct.Suppl. 2:51-0
• Sancar, A., B.M. Kacinski, D.L. Mott, W.D. Rupp 1981. Identification of the uvrC gene product. Proc.Natl.Acad.Sci.USA 78:5450-5454
• Nishimura, A., K. Akiyama, Y. Kohara, K. Horiuchi 1992. Correlation of a subset of the pLC plasmids to the physical map of Escherichia coli K-12. Microbiol.Rev. 56:137-151