W3104Lac      Source of Strain:
J. StadlerOther Designations:
F- Chromosomal Markers: lac-16
, rph-1Strain Comments:
- galT12-- was called gal4 in the Lederberg laboratory. It is the standard gal4 ( galT12) HFT source.
- IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
- rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
- strR = streptomycin resist.
- Stadler, J, EA Adelberg 1972. Temperature dependence of sex-factor maintenance in Escherichia coli K-12. J. Bacteriol. 109(1):447-9.