CGSC Strain#: 3448
Strain Designation: W3104Lac     
Source of Strain: J. Stadler
Other Designations: AB3448
Sex: F-
Chromosomal Markers: lac-16,
galT12,
IN(rrnD-rrnE)1,
rpsL61(strR)
,
rph-1Strain Comments: - galT12-- was called gal4 in the Lederberg laboratory. It is the standard gal4 ( galT12) HFT source.
- IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
- rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
- strR = streptomycin resist.
References: - Stadler, J, EA Adelberg 1972. Temperature dependence of sex-factor maintenance in Escherichia coli K-12. J. Bacteriol. 109(1):447-9.