CGSC Strain#: 6670
Strain Designation: E621=14R21     
Source of Strain: H.C. Wu
Sex: F-
Chromosomal Markers: Δ(gpt-proA)62,
lacY1,
tsx-29,
glnX44(AS)
,
galK2(Oc)
,
lpp-21,
hisG4(Oc)
,
xylA5,
mtl-1,
argE3(Oc)
,
thiE1Strain Comments: - glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- Δ(gpt-proA)62 was formerly called proA2
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
References: - Giam, C.Z., T. Chai, S. Hayashi, H.C. Wu 1984. Prolipoprotein modification and processing in Escherichia coli. A unique secondary structure in prolipoprotein signal sequence for the recognition by glyceryl transferase. Eur.J.Biochem. 141:331-337