CGSC Strain#: 7110
Strain Designation: RK4981      Source of Strain: R.J. Kadner
Sex: F- Chromosomal Markers: [araD139]_B/r, Δ(argF-lac)169, λ^-, flhD5301, non-9, Δ(fruK-yeiR)725(fruA25), gyrA219(NalR), relA1, rpsL150(strR), pyrE41, ΔuhpT2050, rbsR22, metE70, deoC1, gltSo14
Strain Comments:

• The thr-leu region was transduced in from E.coli B.
• [araD139]_B/r-- The thr-leu region was transduced from an E. coli B/r strain (SB3118) in early steps of strain derivation; ara or in some strains ara-leu or thr-leu are from B.
• Δ(argF-lac)169-- extends from mmuP through orfs preceding argF, through lac to mhpD, literally Δ(mmuP-mhpD)169. (Peters et al. 2003 JB 185:2017)
• Δ(argF-lac)169-- from strain Hfr3000 U169 was initially called ΔlacU169 and described as a lacZY mutation until found to include argF and lacI.
• flhD5301-- flagellar defect shown in Komeda and Iino
• Δ(fruK-yeiR)725(fruA25)-- : this fruA25 mutation deletes 29 codons of fruK, fruB and fruBKAp promoter to orf yeiR Peters et al. J.Bact.185:2017.
• rpsL150(strR)-- T to C transition mutation resulting in a K43R amino acid substitution.
• gltSo14-- is Halpern's gltC7.
• Δ(fruK-yeiR)725(fruA25) was formerly called fruA25
• fruA25 = former designation
• NalR = Nalidix.acidResist.
• strR = streptomycin resist.

References:

• Shattuck-Eidens, D.M., R.J. Kadner 1983. Molecular cloning of the uhp region and evidence for a positive activator for expression of the hexose phosphate transport system of Escherichia coli. J.Bacteriol. 155:1062-1070