CGSC Strain#: 7199
Strain Designation: RP523     
Source of Strain: S.D. Cosloy
Sex: F-
Chromosomal Markers: thr-1,
leuB6(Am)
,
fhuA21,
lacY1,
hemB220,
glnX44(AS)
,
e14-,
rfbC1,
glpR200(glpc),
thiE1Strain Comments: - This strain carries an uncharacterized permeability defect which renders it permeable to hemin. (Grown on LB plus 10-40 ug/ml hemin.)
- leuB6(Am)-- Suppressed by serU suppressor (supH)
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- e14--- : Loss of the e14 element makes the strain McrA-.
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- rfbC1 was formerly called rfbD1
- thiE1 was formerly called thi-1
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- glpc = constitutive expr.glp regulon
References: - Li, J.-M., H. Umanoff, R. Proenca, C.S. Russell, S.D. Cosloy 1988. Cloning of the Escherichia coli K-12 hemB gene. J.Bacteriol. 170:1021-1025