CGSC Strain#: 7295
Strain Designation: UNC361045     
Source of Strain: Selby and Sancar
Sex: F-
Chromosomal Markers: thr-1,
araC14,
leuB6(Am)
,
Δ(gpt-proA)62,
lacY1,
tsx-33,
qsr'-0,
glnX44(AS)
,
galK2(Oc)
,
λ-,
mfd-1,
zcf-117::Tn10,
Rac-0,
hisG4(Oc)
,
rfbC1,
mgl-51,
rpsL31(strR)
,
kdgK51,
xylA5,
mtl-1,
argE3(Oc)
,
thiE1Strain Comments: - leuB6(Am)-- Suppressed by serU suppressor (supH)
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- zcf-117::Tn10-- cotransduces ~ 90% with purB. Clockwise of mcrA, between base 1607 and 1608 of sequence AE000215 (S.Garges, pers.comm.).
- zcf-117::Tn10-- : PCR analysis by Nichols et al., 1998, placed the insertion within orf b1160 (elbA) at nucleotide 1,211,084 (26.105 min.).
- Δ(gpt-proA)62 was formerly called proA2
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- rfbC1 was formerly called rfbD1
- thiE1 was formerly called thi-1
- zcf-117::Tn10 was formerly called elbA117::Tn10
- = tetracycline resistant
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
- strR = streptomycin resist.
References: - Selby, C.P., A. Sancar 1993. Molecular mechanism of transcription-repair coupling. Science 260:53-58