CGSC Strain#: 13935
Strain Designation: pLC22-48      Source of Strain:
Sex: F+ Episome/Plasmid: pLC22-48A
   Plasmid Markers/Mutations: thr-1, araC14, leuB6(Am), lacY1, glnX44(AS), galK2(Oc), λ^-, mtl-1, thiE1, galT22, recA56, xylA5, ΔtrpE63, recA
   Plasmid Comment: Mixed clone. Hybridizes with clones 253-5, 119, 146, 450, also has recA ca. 61.5 min. See also pLC22-48B through pLC22-48D.
Chromosomal Markers: thr-1, araC14, leuB6(Am), lacY1, glnX44(AS), galK2(Oc), galT22, λ^-, ΔtrpE63, recA56, xylA5, mtl-1, thiE1
Strain Comments:

• Mixed clone. Hybridizes with Kohara clones 253-255, 119, 146, 450, and carries recA
• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• recA56-- Amino acid substitution Arg 60 Cys
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• ΔtrpE63 was formerly called DE(trpE5)
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation

References:

• Clarke, L., J. Carbon 1979. Selection of specific clones from colony banks by suppression or complementation tests. Methods Enzymol. 68:396-408
• Phillips, T.A., V. Vaughn, P.L. Bloch, F.C. Neidhardt 1987. Gene-protein index of Escherichia coli K-12, edition 2, p.919-966. In F.C. Neidhardt, J.L. Ingraham, K.B. Low, B. Magasanik, M. Schaechter, and H.E. Umbarger (ed.), Escherichia coli and Salmo
• Nishimura, A., K. Akiyama, Y. Kohara, K. Horiuchi 1992. Correlation of a subset of the pLC plasmids to the physical map of Escherichia coli K-12. Microbiol.Rev. 56:137-151
• Nishimura, A., K. Akiyama, Y. Kohara, K. Horiuchi 1992. Correlation of a subset of the pLC plasmids to the physical map of Escherichia coli K-12. Microbiol.Rev. 56:137-151