CGSC Strain#: 7098
Strain Designation: AB1976 mud-     
Source of Strain: Geiger and Speyer
Sex: F-
Chromosomal Markers: araBAD-9,
fhuA21,
Δ(gpt-proA)62,
lacY1 or lacZ4,
tsx-3,
glnX44(AS)
,
galK2(Oc)
,
λ-,
purB93(ts)
,
trpE3(Oc)
,
hisG4(Oc)
,
rfbC1,
rpsL8 or rplL9(L?)
,
malT1(λ
R)
,
mtl-1,
metE46,
thiE1Strain Comments: - glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- purB93(ts)-- has a Ts requirement for adenine and an antimutator phenotype (resistance to MNNG and other alkylating agents).
- Δ(gpt-proA)62 was formerly called proA2
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- purB93(ts) was formerly called mud
- rfbC1 was formerly called rfbD1
- thiE1 was formerly called thi-1
- AS = amber(UAG) suppressor
- LamR = Lambda-resistant
- Oc = ochre(UAA) mutation
- strR = streptomycin resist.
- ts = Temperature Sensitive
References: - Geiger, JR, JF Speyer 1977. A conditional antimutator in E. coli. Mol. Gen. Genet. 153(1):87-97.