CGSC Strain#: 7526
Strain Designation: CAG12178gyrA⁺      Source of Strain: S. McAteer
Sex: F- Chromosomal Markers: λ^-, zfa-723::Tn10, rph-1
Strain Comments:

• CAG12178 was found to be gyrA^- and S.McAteer constructed this gyrA⁺ derivative from CAG12178.
• zfa-723::Tn10-- cotransduces 82% with gyrA. Located by PCR at nucleotide 2,341,772 (50.478 min.) within orf yfaL.
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• = tetracycline resistant

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