CGSC Strain#: 5382
Strain Designation: LS854      Source of Strain: L. Soll
Sex: F- Chromosomal Markers: λ^-, trpA9605(Am), his-85(Am), IN(rrnD-rrnE)1, rpsL136(strR), Δcrp-3, metE70, trpR55
Strain Comments:

• crp3(del) is called crp45 in Sabourin & Beckwith.
• his-85 is called his-29 by Soll.
• trpA9605 and his-85 are amber mutations.
• trpA9605(Am)-- is an amber mutation. It is suppressed by supD32.
• his-85(Am)-- is an amber mutation. It is suppressed by supD32.
• IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
• trpR55-- is trpR2 of Yanofsky. It is a frameshift mutation. Trp levels are indistinguishable from those in trpR deletions or amber mutations (no functional repressor).
• his-85(Am) was formerly called his-29
• Am = amber(UAG) mutation
• strR = streptomycin resist.

References:

• Sabourin, D, J Beckwith 1975. Deletion of the Escherichia coli crp gene. J. Bacteriol. 122(1):338-40.