CGSC Strain#: 6521
Strain Designation: MOB145      Source of Strain: D.O. Wood
Sex: Hfr    Point of Origin#: 1   Map Position: 96.80   Direction of Marker Transfer: Clockwise
Chromosomal Markers: gal-55, LAM, relA1, spoT1, thiE1, hsdR4, opgB202::Tn10
Strain Comments:

• Parent strain, HB94, was derived from a λ^- strain, but was reported to be immune to λ.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• opgB202::Tn10-- cotransduces 34% with serB and 13% with thr and ca. 50% with hsd. Shown by PCR analysis (Nichols et al.) to be within opgB at nucleotide 4,595,002 (99.0 min.).
• opgB202::Tn10 was formerly called
• thiE1 was formerly called thi-1
• = tetracycline resistant

References: