CGSC Strain#: 4316
Strain Designation: PK191      Source of Strain: P. Kahn
Sex: Hfr    Point of Origin#: 66(ColV)   Map Position: 43.80   Direction of Marker Transfer: Clockwise
Chromosomal Markers: Δ(gpt-lac)5, glnX44(AS), λ^-, relA1, thiE1
Strain Comments:

• First ref.(Kahn) below is to parent PK19.
• The deletion was formerly called DE( proB-lac_X111.
• This strain is ColV^- and ColV^R It is an Hfr_V.
• Δ(gpt-lac)5-- is the deletion Δ( pro-lac)_X111 of Jacob and originated in the strain Hfr 3000 X111.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• AS = amber(UAG) suppressor

References:

• Kahn, PL 1968. Isolation of high-frequency recombining strains from Escherichia coli containing the V colicinogenic factor. J. Bacteriol. 96(1):205-14.
• Low, K.B. 1973. Rapid mapping of conditional and auxotrophic mutants of Escherichia coli K-12. J.Bacteriol. 113:798-812