CGSC Strain#: 4427
Strain Designation: W6      Source of Strain: J. Lederberg
Sex: F+ Chromosomal Markers: relA1, spoT1, metB1, rrnB-2, creC510
Strain Comments:

• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• rrnB-2-- : loss of spacer loop sequence
• creC510-- The constitutive phenotype is due to an R77P amino acid substitution
• creC510 was formerly called phoM510
• Const = constitutive

References:

• Lederberg, J., L. Cavalli, E.M. Lederberg 1952. Sex compatibility in Escherichia coli. Genetics 37:720-730
• Gosting, D.C., J. Lederberg 1956. Biotin requirement of cultures derived from E. coli K-12 strain 58. Microbial Genet.Bull. 13:13-14
• Bachmann, BJ 1972. Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol.Rev. 36(4):525-57.