CGSC Strain#: 5168
Strain Designation: G11a1      Source of Strain: H.G. Boman
Sex: Hfr    Point of Origin#: 2A   Map Position: 12.20   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: relA1, spoT1, ilv-299, metB1, ampCp-1
Strain Comments:

• We received feedback that this strain, along with MG1655 and a few of its derivatives that were tested, showed thermotolerance at 48C on LB without NaCl, but not on regular LB.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• ampCp-1-- is a promoter mutation; AmpC⁺ strains carrying this allele produce about 10X the amount of beta lactamase found in wild-type strains. In absence of other amp alleles, use 10-15 ug/ml Amp.
• ampCp-1 was formerly called ampA1

References:

• Eriksson-Grennberg, K.G., H.G. Boman, J.A.T. Jansson, S. Thoren 1965. Resistance of Escherichia coli to penicillins. I. Genetic study of some ampicillin mutants. J.Bacteriol. 90:54-62