CGSC Strain#: 5097
Strain Designation: E486      Source of Strain: Wechsler and Gross
Sex: F- Chromosomal Markers: thr-1, leuB6(Am), fhuA21, dnaE486(ts), lacY1, glnX44(AS), λ^-, rfbC1, thyA6, rpsL67(strR), thiE1, deoC1, met-89
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• thyA6-- : Even with Luria or other complete media, supplement with thymine.
• thyA6-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• strR = streptomycin resist.
• ts = Temperature Sensitive

References:

• Wechsler, JA, JD Gross 1971. Escherichia coli mutants temperature-sensitive for DNA synthesis. Mol. Gen. Genet. 113(3):273-84.
• Gross, J. 1972. Curr.Topics Microbiol.Immunol. 57:39-0