CGSC Strain#: 5508
Strain Designation: Lin 7      Source of Strain: E.C.C. Lin
Sex: Hfr    Point of Origin#: 2A   Map Position: 12.20   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: fhuA2::IS2, ΔphoA8, ompF627(T2R), fadL701(T2R), relA1, glpR2(glp^c), pitA10, spoT1, rrnB-2, mcrB1, creC510
Strain Comments:

• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• rrnB-2-- : loss of spacer loop sequence
• creC510-- The constitutive phenotype is due to an R77P amino acid substitution
• creC510 was formerly called phoM510
• fhuA2::IS2 was formerly called fhuA2
• ::IS2 = Insertion of IS2
• Const = constitutive
• glp^c = constitutive expr.glp regulon
• T2R = T2 Resistant

References:

• Hayashi, H., J.P. Koch, E.C.C. Lin 1964. Active transport of L-a-glycerophosphate in Escherichia coli. J.Biol.Chem. 239:3098-3105
• HAYASHI, S, EC LIN 1965. CAPTURE OF GLYCEROL BY CELLS OF ESCHERICHIA COLI. Biochim. Biophys. Acta 94:479-87.