CGSC Strain#: 5512
Strain Designation: Lin 75     
Source of Strain: E.C.C. Lin
Sex: Hfr
Point of Origin#: 2A
Map Position: 12.20
Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: fhuA22,
ΔphoA8,
ompF627(T2R)
,
fadL701(T2R)
,
relA1,
Δ(malQ-glpR)14(glpc),
glpD19,
pitA10,
spoT1,
rrnB-2,
mcrB1,
creC510Strain Comments: - This is an aerobic glycerol-negative (G3P deHase-negative) derivative of the glpR-deleted (glp-operon-constitutive) strain Lin 72.
- glpD19-- Loss of G-3-P dehydrogenase (aerobic) results in inability of strains to grow on glycerol or glycerol-3-P as sole carbon source.
- glpD19-- Loss of GlpD also results in stasis with gluconeogenic C sources, e.g.succinate. glpA or glpD mutants should be grown on min.salts + 1% glycerol-free casein hydrolysate + 1% glucose.
- spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- rrnB-2-- : loss of spacer loop sequence
- creC510-- The constitutive phenotype is due to an R77P amino acid substitution
- creC510 was formerly called phoM510
- Δ(malQ-glpR)14(glpc) was formerly called malA34
- Δ(malQ-glpR)14(glpc) was formerly called glpR5
- Const = constitutive
- glpc = constitutive expr.glp regulon
- T2R = T2 Resistant
References: - Freedberg, W.B., E.C.C. Lin 1973. Three kinds of controls affecting the expression of the glp regulon in Escherichia coli. J.Bacteriol. 115:816-816
- Freedberg, W.B., E.C.C. Lin 1973. Three kinds of controls affecting the expression of the glp regulon in Escherichia coli. J.Bacteriol. 115:816-816