CGSC Strain#: 5125
Strain Designation: GM31     
Source of Strain: M.G. Marinus
Sex: F-
Chromosomal Markers: thr-1,
araC14,
leuB6(Am)
,
fhuA31,
lacY1,
tsx-78,
glnX44(AS)
,
galK2(Oc)
,
galT22,
λ-,
dcm-6,
hisG4(Oc)
,
rpsL136(strR)
,
xylA5,
mtl-1,
thiE1Strain Comments: - leuB6(Am)-- Suppressed by serU suppressor (supH)
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- dcm-6-- has a Gln to Arg mutation,also a TGG to TGA termination mutation,which results in dcm-vsr message degradation and causes a Vsr- phenotype (Dar 1993).
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
- strR = streptomycin resist.
References: - Marinus, MG 1973. Location of DNA methylation genes on the Escherichia coli K-12 genetic map. Mol. Gen. Genet. 127(1):47-55.