CGSC Strain#: 7566
Strain Designation: DS122      Source of Strain: S. Rahuel-Clermont
Sex: F- Chromosomal Markers: λ^-, ΔgapA12::Cm, IN(rrnD-rrnE)1, rph-1
Strain Comments:

• ΔgapA12::Cm-- : gap/epd deleted strains should be grown on minimal supplemented with casamino acids, Trp, Met, Thi, succinate + glycerol + antibiotic; they do not grow on LB or glucose media.
• IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)

References:

• Seta, F.D., S. Boschi-Muller, M.L. Vignais, G. Branlant 1997. Characterization of Escherichia coli strains with gapA and gapB genes deleted. J.Bacteriol. 179:5218-5221