CGSC Strain#: 5550
Strain Designation: PA340     
Source of Strain: F. Jacob
Sex: F-
Chromosomal Markers: thr-1,
leuB6(Am)
,
fhuA2::IS2,
lacY1,
glnX44(AS)
,
gal-6,
λ-,
gdhA1,
hisG1,
rfbC1,
galP63,
Δ(gltB-gltF)500,
malT1(λ
R)
,
xyl-7,
mtlA2,
ΔargH1,
rplL9(L?)
,
thiE1Strain Comments: - leuB6(Am)-- Suppressed by serU suppressor (supH)
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- hisG1-- This mutation has been reported as either a frameshift or a 36 nt deletion
- Δ(gltB-gltF)500-- :Glt- phenotype in gltB mutant requires presence of a gdhA mutation as well. 0.2% aspartate is a better N-source than glutamate because of uptake(Pahel et al).
- Δ(gltB-gltF)500 was formerly called aspB24
- Δ(gltB-gltF)500 was formerly called aspB21
- Δ(gltB-gltF)500 was formerly called gltB31
- fhuA2::IS2 was formerly called fhuA2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- rfbC1 was formerly called rfbD1
- thiE1 was formerly called thi-1
- xyl-7 was formerly called xylA7
- ::IS2 = Insertion of IS2
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- L? = assignment uncertain
- LamR = Lambda-resistant
References: - Reiner, A.M. 1969. Isolation and mapping of polynucleotide phosphorylase mutants of Escherichia coli. J.Bacteriol. 97:1431-1436
- Berberich, M.A. 1972. A glutamate-dependent phenotype in E. coli K-12: the result of two mutations. Biochem.Biophys.Res.Commun. 47:1498-1503
- Pahel, G, AD Zelenetz, BM Tyler 1978. gltB gene and regulation of nitrogen metabolism by glutamine synthetase in Escherichia coli. J. Bacteriol. 133(1):139-48.
- Castano, I., F. Bastarrachea, A. Covarrubias 1988. gltBDF operon of Escherichia coli. J.Bacteriol. 170:821-827