CGSC Strain#: 5150
Strain Designation: W945 sdh      Source of Strain: U. Henning
Sex: F- Chromosomal Markers: thr-1, glnX44(AS), galK2(Oc), sdh-1, λ^-, rpsL265(strR), xylA5
Strain Comments:

• We don`t think that there is any possibility of tracing the derivation of this strain. Clearly it is not just an sdh^- mutant of W945.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

References:

• Creaghan, IT, JR Guest 1972. Amber mutants of the -ketoglutarate dehydrogenase gene of Escherichia coli K12. J. Gen. Microbiol. 71(2):207-20.