CGSC Strain#: 5548
Strain Designation: JP58      Source of Strain: A.J. Pittard
Sex: F- Chromosomal Markers: glnX44(AS), galK2(Oc), λ^-, rpsL704(strR), xylA5, mtl-1, ilvC7, argE3(Oc), thiE1, tfr-3
Strain Comments:

• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

References:

• Butlin, J.D., G.B. Cox, F. Gibson 1971. Oxidative phosphorylation in Escherichia coli K12. Mutations affecting magnesium ion- or calcium ion-stimulated adenosine triphosphatase. Biochem.J. 124:75-81