CGSC Strain#: 5557
Strain Designation: Lin 118      Source of Strain: E.C.C. Lin
Sex: F- Chromosomal Markers: lacY1 or lacZ4, glnX44(AS), λ^-, glpA24, purF1, fadL701(T2R), rpsL8 or rplL9(L?) or rpsL14, glpD10, pitA10, xyl-7, mtlA2, spoT1, ΔargH1, thiE1
Strain Comments:

• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• purF1-- is a very stable mutation and may be an intragenic deletion.
• glpD10-- : Loss of G-3-P dehydrogenase (aerobic) results in inability of strains to grow on glycerol or glycerol-3-P as sole carbon source.
• glpD10-- : Loss also results in stasis with gluconeogenic C sources, e.g., succinate. glpA or glpD mutants should be grown on min.salts+1% glycerol-free casein hydrolysate+1% glucose
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• xyl-7 was formerly called xylA7
• AS = amber(UAG) suppressor
• strR = streptomycin resist.
• T2R = T2 Resistant

References:

• Kistler, WS, EC Lin 1971. Anaerobic L- -glycerophosphate dehydrogenase of Escherichia coli: its genetic locus and its physiological role. J. Bacteriol. 108(3):1224-34.