CGSC Strain#: 5560
Strain Designation: Lin 188      Source of Strain: E.C.C. Lin
Sex: F- Chromosomal Markers: fhuA2 or fhuA22, glnX44(AS), gal-6, λ^-, hisG1, rpsL8 or rplL9(L?) or rpsL17, malT1(λ^R), glpD10, mtlA2, thiE1, T2R
Strain Comments:

• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• hisG1-- This mutation has been reported as either a frameshift or a 36 nt deletion
• glpD10-- : Loss of G-3-P dehydrogenase (aerobic) results in inability of strains to grow on glycerol or glycerol-3-P as sole carbon source.
• glpD10-- : Loss also results in stasis with gluconeogenic C sources, e.g., succinate. glpA or glpD mutants should be grown on min.salts+1% glycerol-free casein hydrolysate+1% glucose
• T2R-- : selected for resistance to phage T2
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• = strep-resistant
• AS = amber(UAG) suppressor
• LamR = Lambda-resistant

References:

• Cozzarelli, NR, WB Freedberg, EC Lin 1968. Genetic control of L-alpha-glycerophosphate system in Escherichia coli. J. Mol. Biol. 31(3):371-87.