CGSC Strain#: 5575
Strain Designation: Hfr 3000 YA139      Source of Strain: F. Jacob
Sex: Hfr    Point of Origin#: 1   Map Position: 96.80   Direction of Marker Transfer: Clockwise
Chromosomal Markers: panB6, λ^-, e14-, relA1, spoT1, thiE1
Strain Comments:

• This strain is apparently no longer a high-frequency donor.
• e14--- : Loss of the e14 element makes the strain McrA^-.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• thiE1 was formerly called thi-1

References:

• Cronan, JE 1980. Beta-alanine synthesis in Escherichia coli. J. Bacteriol. 141(3):1291-7.