CGSC Strain#: 5583
Strain Designation: N43      Source of Strain: H. Nakamura
Sex: F- Chromosomal Markers: araC14, Δlac-85, acrA1, glnX44(AS), galK2(Oc), rpsL197(strR), malT1(λ^R), xylA5, mtl-1
Strain Comments:

• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• AS = amber(UAG) suppressor
• LamR = Lambda-resistant
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

References:

• Nakamura, H. 1965. Gene-controlled resistance to acriflavine and other basic dyes in Escherichia coli. J.Bacteriol. 90:8-14
• Nakamura, H, T Tojo, J Greenberg 1975. Interaction of the expression of two membrane genes, acrA and plsA, in Escherichia coli K-12. J. Bacteriol. 122(3):874-9.