CGSC Strain#: 5399
Strain Designation: W4032      Source of Strain: J. Lederberg
Sex: Hfr    Point of Origin#: 2A   Map Position: 12.20   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: Δpro-3, Δ(cod-lacI)6, tsx-76, relA1, metB1, creC510
Strain Comments:

• Δpro-3-- is proA or proB.
• Δ(cod-lacI)6-- is Lederberg's lac^-₇₂ from strain W4032. It is lacI^-Z^-Y^-. Feedback, 1999, indicates that it also deletes part or all of the cod operon.
• creC510-- The constitutive phenotype is due to an R77P amino acid substitution
• creC510 was formerly called phoM510
• Δ(cod-lacI)6 was formerly called lac-3
• Δ(cod-lacI)6 was formerly called lac-72(del)
• Δ(cod-lacI)6 was formerly called Δ6
• Const = constitutive

References:

• Luria, S.E., J.N. Adams, R.C. Ting 1960. Transduction of lactose-utilizing ability among strains of E. coli and S. dysenteriae and the properties of the transducing phage particles. Virology 12:348-390
• Cook, A., J. Lederberg 1962. Recombination studies of lactose nonfermenting mutants of Escherichia coli K12. Genetics 47:1335-1353