CGSC Strain#: 5656
Strain Designation: C101a     
Source of Strain: A. Garen
Sex: Hfr
Point of Origin#: 2A
Map Position: 12.20
Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: fhuA22,
ompF627(T2R)
,
fadL701(T2R)
,
relA1,
pitA10,
pst-32,
rrnB-2,
mcrB1,
creC510Strain Comments: - This strain may no longer be Hfr.
- pst-32-- is a mutation in either pstA or pstB
- rrnB-2-- : loss of spacer loop sequence
- creC510-- The constitutive phenotype is due to an R77P amino acid substitution
- creC510 was formerly called phoM510
- pst-32 was formerly called phoT32
- Const = constitutive
- T2R = T2 Resistant
References: - Garen, A., S. Garen 1963. Genetic evidence on the nature of the repressor for alkaline phosphatase in E. coli. J.Mol.Biol. 6:433-438
- Willsky, G.R., R.L. Bennett, M. Malamy 1973. Inorganic phosphate transport in Escherichia coli: involvement of two genes which play a role in alkaline phosphatase regulation. J.Bacteriol. 113:529-539