CGSC Strain#: 5673
Strain Designation: JR28     
Source of Strain: R. Portalier
Sex: F-
Chromosomal Markers: lacY1 or lacZ4,
tsx-64,
glnX44(AS)
,
gal-6,
hisG1,
thyA3,
metC69,
rpsL8 or rplL9(L?)
or rpsL17,
malT1(λ
R)
,
uxuA1Strain Comments: - glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- hisG1-- This mutation has been reported as either a frameshift or a 36 nt deletion
- thyA3-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
- metC69-- was called metC58 by A.L. Taylor.
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- = strep-resistant
- AS = amber(UAG) suppressor
- LamR = Lambda-resistant
References: - Portalier, R., J.M. Robert-Baudouy, F. Stoeber 1972. Mol.Gen.Genet. 118:335-0