CGSC Strain#: 5585
Strain Designation: DF225     
Source of Strain: D. Fraenkel
Sex: F-
Chromosomal Markers: leuB6(Am)
,
fhuA2::IS2,
lacY1,
tsx-1,
glnX44(AS)
,
gal-6,
λ-,
gapA3,
hisG4(Oc)
,
argG6,
rpsL104,
malT1(λ
R)
,
xyl-7,
mtlA2,
metB1Strain Comments: - The gap-3 marker reverts at a relatively high rate.
- leuB6(Am)-- Suppressed by serU suppressor (supH)
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- fhuA2::IS2 was formerly called fhuA2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- xyl-7 was formerly called xylA7
- = strep-resistant
- ::IS2 = Insertion of IS2
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- LamR = Lambda-resistant
- Oc = ochre(UAA) mutation
References: - Hillman, JD, DG Fraenkel 1975. Glyceraldehyde 3-phosphate dehydrogenase mutants of Escherichia coli. J. Bacteriol. 122(3):1175-9.