CGSC Strain#: 7652
Strain Designation: BW26547      Source of Strain: B.L. Wanner
Sex: F- Episome/Plasmid: Lambda (del) recA+
   Plasmid Markers/Mutations:
Chromosomal Markers: Δ(argF-lac)169, ΔrecA635::kan, rph-1, hsdR514, rob-1, creC510
Strain Comments:

• Δ(argF-lac)169-- extends from mmuP through orfs preceding argF, through lac to mhpD, literally Δ(mmuP-mhpD)169. (Peters et al. 2003 JB 185:2017)
• Δ(argF-lac)169-- from strain Hfr3000 U169 was initially called ΔlacU169 and described as a lacZY mutation until found to include argF and lacI.
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• creC510-- The constitutive phenotype is due to an R77P amino acid substitution
• creC510 was formerly called phoM510
• = kanamycin resistant
• Const = constitutive

References:

• Datsenko, KA, BL Wanner 2000. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U.S.A. 97(12):6640-5.