Lin 206      Source of Strain:
Hfr Point of Origin#:
2A Map Position:
12.20 Direction of Marker Transfer:
Counterclockwise Chromosomal Markers: fhuA22
, spoT1Strain Comments:
- glpA9-- Loss of anaerobic G-3-P deHase results in inability of strains to grow on glycerol or glycerol-3-P as sole C-source under anaerobic conditions.
- glpA9-- Loss of GlpA or D also results in stasis with gluconeogenic C sources, e.g., succinate. glpA or glpD mutants should be grown on Min.salts+ 1% glycerol-free casein hydrolysate + 1%glucose
- spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- T2R = T2 Resistant
- Kistler, WS, EC Lin 1971. Anaerobic L- -glycerophosphate dehydrogenase of Escherichia coli: its genetic locus and its physiological role. J. Bacteriol. 108(3):1224-34.