CGSC Strain#: 5680
Strain Designation: MI178      Source of Strain: M. Mergeay
Sex: Hfr    Point of Origin#: 3   Map Position: 6.80   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: thr-31, carA178, relA1, spoT1, metB1
Strain Comments:

• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.

References:

• Mergeay, M, D Gigot, J Beckmann, N Glansdorff, A Pierard 1974. Physiology and genetics of carbamoylphosphate synthesis in Escherichia coli K12. Mol. Gen. Genet. 133(4):299-316.