CGSC Strain#: 5683
Strain Designation: Lin 205     
Source of Strain: E.C.C. Lin
Sex: Hfr
Point of Origin#: 2A
Map Position: 12.20
Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: fhuA22,
ΔphoA8,
ompF627(T2R)
,
glpA24,
fadL701(T2R)
,
relA1,
glpR8(glpc),
glpR7(glpn),
glpD26,
pitA10,
spoT1Strain Comments: - (Reference below is to strain Lin 233, donor of glpR and glpD mutations.)
- glpR8(glpc)-- is a glp-constitutive mutation, obtained as a reversion of the glpR7, non-inducible phenotype.
- glpR7(glpn)-- may or may not be present in this strain.
- glpD26-- Loss of G-3-P dehydrogenase (aerobic) results in inability of strains to grow on glycerol or glycerol-3-P as sole carbon source.
- spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- glpc = constitutive expr.glp regulon
- glpn = non-inducible for glp regulon
- T2R = T2 Resistant
References: - Cozzarelli, NR, WB Freedberg, EC Lin 1968. Genetic control of L-alpha-glycerophosphate system in Escherichia coli. J. Mol. Biol. 31(3):371-87.