CGSC Strain#: 5862
Strain Designation: PK3      Source of Strain: P. Kahn
Sex: Hfr    Point of Origin#: 131(ColV.)   Map Position: 80.13   Direction of Marker Transfer: Clockwise
Chromosomal Markers: thr-1, leuB6(Am), secA215, fhuA21, lacY1, glnX44(AS), λ^-, rfbC1, thiE1
Strain Comments:

• This is a ColV Hfr. It is ColE1 Resistant, and ColV Resistant and probably ColV⁺.
• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• rfbC1 was formerly called rfbD1
• secA215 was formerly called secA15
• secA215 was formerly called azi-15
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor

References:

• KAHN, P, DR HELINSKI 1964. RELATIONSHIP BETWEEN COLICINOGENIC FACTORS E1 AND V AND AN F FACTOR IN ESCHERICHIA COLI. J. Bacteriol. 88:1573-9.
• Kahn, PL 1968. Isolation of high-frequency recombining strains from Escherichia coli containing the V colicinogenic factor. J. Bacteriol. 96(1):205-14.