CGSC Strain#: 14086
Strain Designation: pLC23-30C     
Source of Strain: Sex: F+
Episome/Plasmid: pLC23-30C
Plasmid Markers/Mutations: thr-1,
araC14,
leuB6(Am)
,
lacY1,
glnX44(AS)
,
galK2(Oc)
,
λ-,
mtl-1,
thiE1,
galT22,
recA56,
xylA5,
ΔtrpE63,
nhoA ,
rrnG(op) Plasmid Comment: Reported to have nhoA by Yamamura et al. 2000. Hybridizes with Kohara clones 276-280, 404, and 532. Carries rrnG at 58.7. See also pLC23-30A, pLC23-30B and pLC23-30D.
Chromosomal Markers: thr-1,
araC14,
leuB6(Am)
,
lacY1,
glnX44(AS)
,
galK2(Oc)
,
galT22,
λ-,
ΔtrpE63,
recA56,
xylA5,
mtl-1,
thiE1Strain Comments: - leuB6(Am)-- Suppressed by serU suppressor (supH)
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- recA56-- Amino acid substitution Arg 60 Cys
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- ΔtrpE63 was formerly called DE(trpE5)
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
References: - Clarke, L, J Carbon 1976. A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome. Cell 9(1):91-9.
- An, G., J.S. Lee, J.D. Friesen 1982. Evidence for an internal promoter preceding tufA in the str operon of Escherichia coli. J.Bacteriol. 149:548-553
- Nishimura, A., K. Akiyama, Y. Kohara, K. Horiuchi 1992. Correlation of a subset of the pLC plasmids to the physical map of Escherichia coli K-12. Microbiol.Rev. 56:137-151
- Yamamura, E., M. Sayama, M. Kakikawa, M. Mori, A. Taketo, K. Kodaira 2000. Purification and biochemical properties of an N-hydroxyarylamine O-acetyltransferase from Escherichia coli. Biochim.Biophys.Acta 1475:10-16