CGSC Strain#: 14142
Strain Designation: pLC2-20B      Source of Strain:
Sex: F+ Episome/Plasmid: pLC2-20B
   Plasmid Markers/Mutations: thr-1, araC14, leuB6(Am), lacY1, glnX44(AS), galK2(Oc), λ^-, mtl-1, thiE1, galT22, recA56, xylA5, ΔtrpE63, plsB , malB , dgkA , lexA , dnaB , qor , ubiA , ubiC
   Plasmid Comment: Mixed clone. Hybridizes with Kohara clones 633-6 at c. 91.5 min. Carries adk ca. 10.7 min. All other genes included are located between 91.35 and 91.9 minutes. See also pLC2-20A.
Chromosomal Markers: thr-1, araC14, leuB6(Am), lacY1, glnX44(AS), galK2(Oc), galT22, λ^-, ΔtrpE63, recA56, xylA5, mtl-1, thiE1
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• recA56-- Amino acid substitution Arg 60 Cys
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• ΔtrpE63 was formerly called DE(trpE5)
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation

References:

• Clarke, L, J Carbon 1976. A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome. Cell 9(1):91-9.
• Lightner, V.A., T.J. Larson, P. Tailleur, G.D. Kantor, C.H.R. Raetz, R.M. Bell, P. Modrich 1980. Membrane phospholipid synthesis in Escherichia coli. Cloning of a structural gene (plsB) of the sn-glycerol-3-phosphate acyltransferase. J.Biol.Chem. 255:
• Nishimura, A., K. Akiyama, Y. Kohara, K. Horiuchi 1992. Correlation of a subset of the pLC plasmids to the physical map of Escherichia coli K-12. Microbiol.Rev. 56:137-151