CGSC Strain#: 7861
Strain Designation: BW5328/pAH123      Source of Strain: B.L. Wanner
Sex: F- Episome/Plasmid: pAH123
   Plasmid Markers/Mutations: LAMcI857(ts), repA101(ts), oriR101, λp_R, bla(ApR), int_φ80, int$_{\Phi 80}$
   Plasmid Comment: helper CRIM
Chromosomal Markers: Δ(argF-lac)169, recA1, rpoS396(Am), rph-1, hsdR514, rob-1, creC510
Strain Comments:

• Also Ref. Khlebnikov et al. In Press Microbiology 2001.
• Δ(argF-lac)169-- extends from mmuP through orfs preceding argF, through lac to mhpD, literally Δ(mmuP-mhpD)169. (Peters et al. 2003 JB 185:2017)
• Δ(argF-lac)169-- from strain Hfr3000 U169 was initially called ΔlacU169 and described as a lacZY mutation until found to include argF and lacI.
• recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• creC510-- The constitutive phenotype is due to an R77P amino acid substitution
• creC510 was formerly called phoM510
• Am = amber(UAG) mutation
• Const = constitutive
• ApR = ampicillin resistant
• ts = Temperature Sensitive

References:

• Haldimann, A, BL Wanner 2001. Conditional-replication, integration, excision, and retrieval plasmid-host systems for gene structure-function studies of bacteria. J. Bacteriol. 183(21):6384-93.